Abstract

Research Article

Anticancer activity of novel surface functionalized metal doped hydroxyapatite

Saheli Pradhan*, Shouvik Mitra and Arunava Goswami

Published: 03 June, 2020 | Volume 4 - Issue 1 | Pages: 021-026

In this manuscript it has been described a novel synthesis of mercury doped hydroxyapatite (Hap) and its application on human liver carcinoma cell line (Hep G2) and human lung fibroblast carcinoma cell line (MRC 5). Nano-sized hydroxyapatite doped with Hg2+ was synthesized by a solution based chemical method along with mercury ion. The surface of nanoparticle of mercury doped hydroxyapatite (MHAp) was functionalized by using phosphonomethyl iminodiacetic acid (PMIDA) for making it stable as dispersed phase with negative zeta potential. Surface functionalization was confirmed by FTIR measurements. Crystalline nature, morphology and surface topology were studied by powder XRD, FESEM and AFM measurements. Particle size of the well dispersed sample was obtained by HRTEM image. The studies on cell viability of Hep G2 and MRC 5 cell in presence of mercury doped HAp nanoparticle (MHAp) were determined through WST assay. It was observed that nanocomposite exhibited a site specific action towards MRC 5 cell lines along with reduction of toxicity toward normal cells.

Read Full Article HTML DOI: 10.29328/journal.apps.1001023 Cite this Article Read Full Article PDF

Keywords:

Hydroxyapatite; Nanocomposite; Surface grafting; Cytotoxicity; Cell viability

References

  1. Kothapalli CR, Shaw MT, Wei M. Biodegradable HA-PLA Porous Scaffolds: Effect of Nano-Sized Filler Content on Composite Properties. Acta Biomater. 2005; 1: 653-662. PubMed: https://pubmed.ncbi.nlm.nih.gov/16701846/
  2. Wang H, Li Y, Zuo Y, Li J, Ma S, et al. Biocompatibility and osteogenesis of biomimetic nano-hydroxyapatite/polyamide composite scaffolds for bone tissue engineering. Biomaterials. 2007; 28: 3338-3348. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/17481726
  3. Pramanik N, Tarafdar A, Pramanik P. Capping agent-assisted synthesis of nanosized hydroxyapatite: Comparative studies of their physicochemical properties. J Mater Process Technol. 2007; 184: 131-138.
  4. Wan YZ, Huang Y, Yuan CD, Raman S, Zhu Y, et al. Biomimetic synthesis of hydroxyapatite/bacterial cellulose nanocomposites for biomedical applications. Mater Sci Eng C. 2007; 27: 855-864.
  5. Marques AP, Reis RL. Hydroxyapatite reinforcement of different starch-based polymers affects osteoblast-like cells adhesion/spreading and proliferation. Mater Sci Eng C. 2005; 25: 215-229.
  6. Converse GL, Yue W, Roeder RK. Processing the tensile properties of hydroxyapatite-whisker-reinforced polyetheretherketone. Biomaterials. 2007; 28: 927-935. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/17113143
  7. Bacon GE, Bacon PJ, Griffiths RK. Neutron diffraction studies of lumbar vertebrae. J Anat. 1979; 128: 277-283. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/438090
  8. Xu X, Chen X, Liu A, Hong Z, Jing X. Electrospun poly(l-lactide)-grafted hydroxyapatite/poly(l-lactide) nanocomposite fibers. Eur. Polym. J. 2007; 43: 3187-3196.
  9. Chen M, Tan J, Lian Y, Liu D. Preparation of Gelatin coated hydroxyapatite nanorods and the stability of its aqueous colloidal. Appl Surf Sci. 2008; 254: 2730-2735.
  10. Kim S, Kim SS, Lee SH, Ahn SE, Gwak SJ, et al. In vivo bone formation from human embryonic stem cell-derived osteogenic cells in poly(d,l-lactic-co-glycolic acid)/hydroxyapatite composite scaffolds. Biomaterials 2008; 29: 1043-1053. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18023477
  11. W OC, Hull JR. Surface modification of nanophase hydroxyapatite with chitosan. Mater Sci Eng C. 2008; 28: 434-437.
  12. Boanini E, Torricelli P, Gazzano M, Giardino R, Bigi A. Nanocomposites of hydroxyapatite with aspartic acid and glutamic acid and their interaction with osteoblast-like cells. Biomaterials. 2006; 27: 4428-4433. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/16682075
  13. Mohapatra S, Pramanik N, Ghosh SK, Pramanik P. Design of Superparamagnetic Iron Oxide Nanoparticle for Purification of Recombinant Proteins. J Nanosci Nanotechnol. 2006; 6 : 823-829.
  14. Pramanik N, Mohapatra S, Pramanik P, Bhargava P. Processing and Properties of Nano-Hydroxyapatite(n-HAp)/ Poly(Ethylene-Co-Acrylic Acid)(EAA) Composite Using a Phosphonic Acid Coupling Agent for Orthopedic Applications. J Am Ceram Soc. 2007; 90: 369-375.
  15. Ikemura K, Tay FR, Nishiyama N, Pashley DH, Endo T. Design of new phosphonic acid monomers for dental adhesives--synthesis of (meth) acryloxyalkyl 3-phosphonopropionates and evaluation of their adhesion-promoting functions. Dent Mater J. 2006; 25: 566-575. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/17076329
  16. Andrea SCD, Fadeev AY. Covalent Surface Modification of Calcium Hydroxyapatite Using n-Alkyl- and n-Fluoroalkylphosphonic Acids. Langmuir. 2003; 19: 7904-7910.
  17. Nancollas GH, Tang R, Phipps RJ, Henneman Z, Gulde S,et al. Novel insights into actions of bisphosphonates on bone: Differences in interactions with hydroxyapatite. Bone. 2006; 38: 617-627. PubMed: https://pubmed.ncbi.nlm.nih.gov/16046206
  18. Ganguli A, Steward C, Butler SL, Philips GJ, Meikle ST, et al. Bacterial adhesion to bisphosphonate coated hydroxyapatite. J Mater Sci Mater Med. 2005; 16 : 283-287. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/15803271
  19. Boanini E, Torricelli P, Gazzano M, Giardino R, Bigi A. Alendronate-hydroxyapatite nanocomposites and their interaction with osteoclasts and osteoblast-like cells. Biomaterials. 2008; 29 : 790-796. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/18022226
  20. Leu CT, Luegmayr E, Freedman LP, Rodan GA, Reszka AA. Relative binding affinities of bisphosphonates for human bone and relationship to antiresorptive efficacy. Bone. 2006; 38: 628-636. PubMed: https://pubmed.ncbi.nlm.nih.gov/16185944
  21. Kim SH, Jeong JH, Chun KW, Park TG. Target-Specific Cellular Uptake of PLGA Nanoparticles Coated with Poly(l-lysine)−Poly(ethylene glycol)−Folate Conjugate. Langmuir. 2005; 21: 8852-8857. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/16142970
  22. Ennis BW, Lippman ME, Dickson RB. The EGF receptor system as a target for antitumor therapy. Cancer Invest. 1991; 9: 553-562. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/1933488
  23. Leamon CP, Reddy JA, Vetzel M, Dorton R, Westrick E, et al. Folate Targeting Enables Durable and Specific Antitumor Responses from a Therapeutically Null Tubulysin B Analogue. Cancer Res. 2008; 68: 9839-9844. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/19047164
  24. Hart A. Antibacterial activity of phenylmercuric nitrate in zinc sulphate and adrenaline eye drops. J Pharm Pharmacol. 1973: 25: 507-508. PubMed: https://www.ncbi.nlm.nih.gov/pubmed/4146598
  25. Barr FS, Collins GF, Moore GW. Evaluation of the antimicrobial activity of a phenylmercuric acetate-bithionol topical spray. Vet Med Small Anim Clin. 1966; 11: 1083.
  26. Pramanik N, Mohapatra S, Bhargava P, Pramanik P. Chemical synthesis and characterization of hydroxyapatite (HAp)-poly (ethylene co vinyl alcohol) (EVA) nanocomposite using a phosphonicacid coupling agent for orthopedic applications. Mater Sci Eng C. 2009: 29: 228-236.
  27. Owen-Szcaub LB. Soluble Fas and Cancer. Clin. Cancer Res. 2001: 7: 1108-1109.

Figures:

Figure 1

Figure 1

Figure 1

Figure 2

Figure 1

Figure 3

Figure 1

Figure 4

Figure 1

Figure 5

Figure 1

Figure 6

Figure 1

Figure 7

Figure 1

Figure 8

Similar Articles

  • Anticancer activity of novel surface functionalized metal doped hydroxyapatite
    Saheli Pradhan*, Shouvik Mitra and Arunava Goswami Saheli Pradhan*,Shouvik Mitra,Arunava Goswami. Anticancer activity of novel surface functionalized metal doped hydroxyapatite. . 2020 doi: 10.29328/journal.apps.1001023; 4: 021-026
  • Cytotoxic Effects of Aminotriles with Bioactive Potential: An Integrative Review
    Paola da Costa Vieira, Lettícia Tenório Cavalcanti, Hélida Maravilha Dantas e Sousa Almeida and Igor de Sousa Oliveira and Sávio Benvindo Ferreira* Paola da Costa Vieira, Lettícia Tenório Cavalcanti, Hélida Maravilha Dantas e Sousa Almeida, Igor de Sousa Oliveira and Sávio Benvindo Ferreira*. Cytotoxic Effects of Aminotriles with Bioactive Potential: An Integrative Review. . 2023 doi: 10.29328/journal.apps.1001040; 7: 022-027

Recently Viewed

  • Difference between conventional and modern methods for examination of fingerprints
    Ambati Ramesh Babu* Ambati Ramesh Babu*. Difference between conventional and modern methods for examination of fingerprints. J Forensic Sci Res. 2021: doi: 10.29328/journal.jfsr.1001025; 5: 037-040
  • Characterization and virulence determination of Colletotrichum kahawae isolates from Gidami, Western Ethiopia
    Zenebe W*, Daniel T and Weyessa G Zenebe W*,Daniel T,Weyessa G. Characterization and virulence determination of Colletotrichum kahawae isolates from Gidami, Western Ethiopia. J Plant Sci Phytopathol. 2021: doi: 10.29328/journal.jpsp.1001054; 5: 004-013
  • Statistical Mathematical Analysis of COVID-19 at World Level
    Marín-Machuca Olegario*, Carlos Enrique Chinchay-Barragán, Moro-Pisco José Francisco, Vargas-Ayala Jessica Blanca, Machuca-Mines José Ambrosio, María del Pilar Rojas-Rueda and Zambrano-Cabanillas Abel Walter Marín-Machuca Olegario*, Carlos Enrique Chinchay-Barragán, Moro-Pisco José Francisco, Vargas-Ayala Jessica Blanca, Machuca-Mines José Ambrosio, María del Pilar Rojas-Rueda, Zambrano-Cabanillas Abel Walter. Statistical Mathematical Analysis of COVID-19 at World Level. Int J Phys Res Appl. 2024: doi: 10.29328/journal.ijpra.1001082; 7: 040-047
  • Rida Herbal Bitters Improve Cardiovascular Function in High-fat Diet/Streptozotocin-induced Diabetic Rats
    Folasade Omobolanle Ajao*, Damilola Ayodeji Balogun, Marcus Olaoy Iyedupe, Ayobami Olagunju, Esther Oparinde, Luqman Adeniji and Victor Abulude and Funmilayo Elizabeth Olaleye Folasade Omobolanle Ajao*, Damilola Ayodeji Balogun, Marcus Olaoy Iyedupe, Ayobami Olagunju, Esther Oparinde, Luqman Adeniji, Victor Abulude and Funmilayo Elizabeth Olaleye. Rida Herbal Bitters Improve Cardiovascular Function in High-fat Diet/Streptozotocin-induced Diabetic Rats. J Cardiol Cardiovasc Med. 2024: doi: 10.29328/journal.jccm.1001177; 9: 044-051
  • Antibacterial Screening of Lippia origanoides Essential Oil on Gram-negative Bacteria
    Rodrigo Marcelino Zacarias de Andrade, Bernardina de Paixão Santos, Roberson Matteus Fernandes Silva, Mateus Gonçalves Silva*, Igor de Sousa Oliveira, Sávio Benvindo Ferreira and Rafaelle Cavalcante Lira Rodrigo Marcelino Zacarias de Andrade, Bernardina de Paixão Santos, Roberson Matteus Fernandes Silva, Mateus Gonçalves Silva*, Igor de Sousa Oliveira, Sávio Benvindo Ferreira, Rafaelle Cavalcante Lira. Antibacterial Screening of Lippia origanoides Essential Oil on Gram-negative Bacteria. Arch Pharm Pharma Sci. 2024: doi: 10.29328/journal.apps.1001053; 8: 024-028.

Read More

Most Viewed

Read More

Help ?