Abstract

Research Article

Next Generation Tools in mRNA Purification: The Role of Continuous Raman Spectroscopy Testing with Pretreatment of the Sample

Luisetto M*, Nili B Ahmadabadi, Khaled Edbey and Oleg Yurevich Latyshev

Published: 29 March, 2024 | Volume 8 - Issue 1 | Pages: 021-023

In the biopharmaceutical production field, the purification process is a crucial step in order to obtain Drugs with an impurity profile according to the regulatory agency requirement.
The aim of this work is to verify some relevant and recent literature and after analysis to submit to the researcher new Solutions in order to improve global safety and the toxicological profile: Submit a project related to the continuous testing of the purified materials using Raman spectroscopy – with pre-treatment of the sample: using solvents.
Nanolipis Payload of Biopharmaceutical is not efficiently detected by direct Raman spectroscopy allowed by the regulatory agency for PAT process analytical technology.

Read Full Article HTML DOI: 10.29328/journal.apps.1001052 Cite this Article Read Full Article PDF

Keywords:

mRNA; Purifications; Chromatography; Separations; Columns; Resins; Monoliths; Activated carbon; Raman spectroscopy; Pre-treatment of sample; Continuous testing systems

References

  1. Campra P. Almeria University https://www.researchgate.net/publication/355979001_DETECTION_OF_GRAPHENE_IN_COVID19_VACCINES-
  2. Luisetto M, Ahmadabadi NB, Ettarhouni ZO, Edbey K, Latyshev OY. Monoliths in the mRNA Vaccine Purification Process the Silica Resin and Other Composite Materials: The Carbon Content. Int Case Rep Jour. 2022; 2(7):1-23.
  3. Luisetto M, Edbey K, Tarro G, Ahmadabadi NB, Khan FA. Activated Charcoal and Derivate Materials in Drugs and Biopharmaceutical Purification: Impurity Aspects. J Mater Sci Nanotechnol. 2023; 11(1):105.
  4. Cipelli RB, Giovannini F, Dark GP. Field Microscopic Analysis on the Blood of 1,006 Symptomatic Persons After Anti-COVID mRNA Injections from Pfizer/BioNtech or Moderna International Journal of Vaccine Theory, Practice, and Research. 2022-08-12. Journal article DOI: 10.56098/ijvtpr.v2i2.47 CONTRIBUTORS:
  5. Luisetto M. Raman (Rs) Spectroscopy for Biopharmaceutical Quality Control and PAT. Raw Material - Final Products: the Nanolipids Effect on Signal Intensity. Regulatory and Toxicological Aspects. Med & Analy Chem Int J. 2022; 6(1): 000175.
  6. Vanden-Hehir S, Tipping WJ, Lee M, Brunton VG, Williams A, Hulme AN. Raman Imaging of Nanocarriers for Drug Delivery. Nanomaterials (Basel). 2019 Mar 3;9(3):341. doi: 10.3390/nano9030341. PMID: 30832394; PMCID: PMC6474004.
  7. Activated charcoal BP monography.
  8. Luisetto WM. Amorphous substance (activated charcoal) and other impurities in API manufacturing process. Pharmaceutical, toxicological and regulatory implication. 2023.
  9. Robert OY. Scanning & Transmission Electron Microscopy Reveals Graphene Oxide in CoV-19 Vaccines 2021. February 5th, Updated September 11th, 2021. www.drrobertyoung.com

Figures:

Similar Articles

Recently Viewed

  • Unusual and severe peripartum cardiomyopathy: A case report
    Lamanna B*, Vinciguerra M, Crupano FM, Cicinelli R, Cicinelli E and Vimercati A Lamanna B*,Vinciguerra M,Crupano FM,Cicinelli R,Cicinelli E,Vimercati A. Unusual and severe peripartum cardiomyopathy: A case report. J Cardiol Cardiovasc Med. 2021: doi: 10.29328/journal.jccm.1001116; 6: 040-043
  • Extracorporeal Membrane Oxygenation in Acute Aluminum Phosphide (AlP) Poisoning
    Oussama Ssouni*, Latifa Oualili, Tarek Dendane, Amine Ali Zeggwagh and Khalid Abidi Oussama Ssouni*, Latifa Oualili, Tarek Dendane, Amine Ali Zeggwagh, Khalid Abidi. Extracorporeal Membrane Oxygenation in Acute Aluminum Phosphide (AlP) Poisoning. Arch Surg Clin Res. 2023: doi: 10.29328/journal.ascr.1001071; 7: 024-034
  • Antibacterial Screening of Lippia origanoides Essential Oil on Gram-negative Bacteria
    Rodrigo Marcelino Zacarias de Andrade, Bernardina de Paixão Santos, Roberson Matteus Fernandes Silva, Mateus Gonçalves Silva*, Igor de Sousa Oliveira, Sávio Benvindo Ferreira and Rafaelle Cavalcante Lira Rodrigo Marcelino Zacarias de Andrade, Bernardina de Paixão Santos, Roberson Matteus Fernandes Silva, Mateus Gonçalves Silva*, Igor de Sousa Oliveira, Sávio Benvindo Ferreira, Rafaelle Cavalcante Lira. Antibacterial Screening of Lippia origanoides Essential Oil on Gram-negative Bacteria. Arch Pharm Pharma Sci. 2024: doi: 10.29328/journal.apps.1001053; 8: 024-028.
  • Systemic Lupus Erythematosus and Depression Overview
    Ahmed Shaaban*, Nourelhouda Said and Hoda Afifi Ahmed Shaaban*,Nourelhouda Said,Hoda Afifi. Systemic Lupus Erythematosus and Depression Overview. Insights Depress Anxiety. 2024: doi: 10.29328/journal.ida.1001039; 8: 001-004
  • Cytotoxic Effects of Aminotriles with Bioactive Potential: An Integrative Review
    Paola da Costa Vieira, Lettícia Tenório Cavalcanti, Hélida Maravilha Dantas e Sousa Almeida and Igor de Sousa Oliveira and Sávio Benvindo Ferreira* Paola da Costa Vieira, Lettícia Tenório Cavalcanti, Hélida Maravilha Dantas e Sousa Almeida, Igor de Sousa Oliveira and Sávio Benvindo Ferreira*. Cytotoxic Effects of Aminotriles with Bioactive Potential: An Integrative Review. Arch Pharm Pharma Sci. 2023: doi: 10.29328/journal.apps.1001040; 7: 022-027

Read More

Most Viewed

Read More

Help ?